| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
27/06/1995 |
|
Data da última atualização: |
27/06/1995 |
|
Autoria: |
BADILLO FELICIANO, J.; REYESSOTO, I.; BEAVER, J. S. |
|
Título: |
A comparison of yields of common beans at physiological and harvest maturity. |
|
Ano de publicação: |
1985 |
|
Fonte/Imprenta: |
J. Agric. Univ. Porto Rico, v.69, n.1, p.19-24, 1985. |
|
Idioma: |
Inglês |
|
Thesagro: |
Feijão. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00429naa a2200145 a 4500
001 1606298
005 1995-06-27
008 1985 bl --- 0-- u #d
100 1 $aBADILLO FELICIANO, J.
245 $aA comparison of yields of common beans at physiological and harvest maturity.
260 $c1985
650 $aFeijão
700 1 $aREYESSOTO, I.
700 1 $aBEAVER, J. S.
773 $tJ. Agric. Univ. Porto Rico$gv.69, n.1, p.19-24, 1985.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
02/08/2017 |
|
Data da última atualização: |
17/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
ALMEIDA, V. M.; LUZ, G. A.; MARTINS, P. P.; GOMES, M. F. C.; COSTA, M. F.; LIMA, P. S. da C.; VALENTE, S. E. S. |
|
Afiliação: |
UFPI; UFPI; UFPI; UFPI; UFPI; PAULO SARMANHO DA COSTA LIMA, CPAMN; UFPI. |
|
Título: |
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v.16, n. 3: gmr16039724, 2017. |
|
Páginas: |
7 p. |
|
DOI: |
http://dx.doi.org/10.4238/gmr16039724 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits. |
|
Palavras-Chave: |
DNA isolation; Isolamento do DNA; Mangabeira; Reação em cadeia da polimerase. |
|
Thesaurus NAL: |
polymerase chain reaction. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162322/1/ArtigoPauloSarmanhoGMRJul2017.pdf
|
|
Marc: |
LEADER 02098naa a2200277 a 4500
001 2073486
005 2022-05-17
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr16039724$2DOI
100 1 $aALMEIDA, V. M.
245 $aComparison of eight methods to isolate genomic DNA from Hancornia speciosa.$h[electronic resource]
260 $c2017
300 $a7 p.
520 $aMangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits.
650 $apolymerase chain reaction
653 $aDNA isolation
653 $aIsolamento do DNA
653 $aMangabeira
653 $aReação em cadeia da polimerase
700 1 $aLUZ, G. A.
700 1 $aMARTINS, P. P.
700 1 $aGOMES, M. F. C.
700 1 $aCOSTA, M. F.
700 1 $aLIMA, P. S. da C.
700 1 $aVALENTE, S. E. S.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv.16, n. 3: gmr16039724, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
